Substance P Hydrolysis by Human Serum Cholinesterase
نویسندگان
چکیده
منابع مشابه
Substance P hydrolysis by human serum cholinesterase.
Highly purified human serum cholinesterase (EC 3.1.1.8, also known as pseudocholinesterase and butyrylcholinesterase) had peptidase activity toward substance P. Digestion of substance P was monitored by high performance liquid chromatography, which separated three product peptides. The cleavages occurred sequentially. The first peptide to appear as Arg1-Pro2. The Km for this hydrolysis was 0.3 ...
متن کاملStructure of human serum cholinesterase.
Human cholinesterase has recently been sequenced and cloned. It is a glycoprotein of 4 identical subunits, each subunit containing 9 carbohydrate chains and 3.5 disuljide bonds. Protein folding is likely to be very similar in human cholinesterase and Torpedo acetylcholinesterase. The cholinesterases have no significant sequence homology with the serine proteases and seem to belong to a separate...
متن کاملOxime reactivation of diethylphosphoryl human serum cholinesterase.
Reactivation of diethyl p-nitrophenyl phosphate inhibited human serum cholinesterase by pyridine-Z-aldoxime methiodide and isonitrosoacetophenone has been investigated as a function of reactivator concentration, pH, and temperature in boric acid-borax buffer and in salt solution. Constants shown were the dissociation constants of the diethylphosphoryl cholinesterase reactivator complex for the ...
متن کاملPurification and properties of human serum cholinesterase.
Cholinesterase was purified from human serum by a three-stage procedure involving chromatography on DEAE-cellulose at pH4.0, an electrofocusing technique and gel filtration on Sephadex G-200. The final product was purified 13000-fold with a yield of 54%, and only one protein and one cholinesterase band could be demonstrated by polyacrylamide-disc electrophoresis. The catalytic properties appear...
متن کاملMolecular heterogeneity of human serum cholinesterase.
s of Papers, 160th Nat. Meeting, American Chemical Society, BIOL 185, Sept. 14-18, 1970. 25. Des, P. K., and Liddell, J., Purification and properties of human serum cholinesterase. Biochem. J. 116, 875 (1970). 26. Kalow, W., and Lindsay, H. A., A comparison of optical andinanometric methods for the assay of human serum cholin-esterase. Can. J. Biochem. Physiol. 33, 568 (1955). 27. Marke...
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ژورنال
عنوان ژورنال: Journal of Neurochemistry
سال: 1982
ISSN: 0022-3042,1471-4159
DOI: 10.1111/j.1471-4159.1982.tb04707.x